Difference between revisions of "Part:BBa K4907034"

 
 
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<partinfo>BBa_K4907034 short</partinfo>
 
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===Biology===
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Bacterial cellulose (BC) considered as an important biopolymer because of its many more particular properties compared to plant cellulose. In cellulose-producing bacterial strains, most prominent of which is <i>Gluconoacetobacter hansenii</i>, BC biosynthesis is orchestrated by four genes; namely A, B, C, and D comprising a bacterial cellulose synthase (bcs) operon, preceded by two "regulatory" fragments. In some studies, it noted that BcsA and BcsB are two catalytic subunits that are present in all Bcs enzymes. Only bcsAB fragments are enough for effective <i>in vitro</i> BC production.When <i>bcsA</i> is activated by c-di-GMP, it incorporates glucose units into a cellulose chain in the cytoplasm using UDP glucose as a substrate. BcsB guides the glucan chain through the periplasm (1,2).
  
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===Reference===
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===Usage and Biology===
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#E. Sajadi <i>et al</i>., Increased cellulose production by heterologous expression of <i>bcsA</i> and B genes from <i>Gluconacetobacterxylinus</i> in <i>E. coli</i> Nissle 1917. <i>Bioprocess. Biosyst. Eng.</i> <b>42</b>, 2023-2034 (2019).
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#S. S. Al-Janabi <i>et al</i>., Stable, efficient, and cost-effective system for the biosynthesis of recombinant bacterial cellulose in <i>Escherichia coli</i> DH5α platform. J. Genet. <i>Eng. Biotechnol.</i> <b>20</b>, 90 (2022).
  
 
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Latest revision as of 22:47, 10 October 2023


bcsB

Biology

Bacterial cellulose (BC) considered as an important biopolymer because of its many more particular properties compared to plant cellulose. In cellulose-producing bacterial strains, most prominent of which is Gluconoacetobacter hansenii, BC biosynthesis is orchestrated by four genes; namely A, B, C, and D comprising a bacterial cellulose synthase (bcs) operon, preceded by two "regulatory" fragments. In some studies, it noted that BcsA and BcsB are two catalytic subunits that are present in all Bcs enzymes. Only bcsAB fragments are enough for effective in vitro BC production.When bcsA is activated by c-di-GMP, it incorporates glucose units into a cellulose chain in the cytoplasm using UDP glucose as a substrate. BcsB guides the glucan chain through the periplasm (1,2).

Reference

  1. E. Sajadi et al., Increased cellulose production by heterologous expression of bcsA and B genes from Gluconacetobacterxylinus in E. coli Nissle 1917. Bioprocess. Biosyst. Eng. 42, 2023-2034 (2019).
  2. S. S. Al-Janabi et al., Stable, efficient, and cost-effective system for the biosynthesis of recombinant bacterial cellulose in Escherichia coli DH5α platform. J. Genet. Eng. Biotechnol. 20, 90 (2022).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 687
    Illegal NheI site found at 802
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1690
    Illegal BamHI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1441
    Illegal AgeI site found at 40
    Illegal AgeI site found at 1000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1179