Difference between revisions of "Part:BBa K4960015"

 
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<partinfo>BBa_K4960015 short</partinfo>
 
<partinfo>BBa_K4960015 short</partinfo>
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===Properties===
  
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PVC16 is a part of PVC Sheath Terminator.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Possessing a central core and six arms.
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Six helices from the Pvc16 hexamer completely seal the central channel of the tube, leaving a narrow pore with a diameter of 7 A.
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Each Pvc16 monomer exhibits a dumbbell-like structure. The N-terminal domain (NTD) and C-terminal domain (CTD) is connected by a middle linker (MD).
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The main function is to be as a tube terminator.
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Each Pvc16-NTD interacts with three copies of Pvc1.
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In the absence of Pvc16 (DPvc16), the baseplate and tube could still form, but not the sheath, and the length of the tube appears to be much larger.
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A minor role of Pvc16-CTD in stabilizing the sheath-tube complex at the capping end.
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The binding of Pvc16 onto the PVC complex might not require the help of outer sheath and the capping of Pvc16 on the distal Pvc1 ring might set the signal to initiate the sheath loading.
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After the tube reaches optimal length (likely controlled by the tape measure protein Pvc14), Pvc16 interacts with the topmost inner tube layer to terminate the tube elongation.
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4960015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960015 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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===References===
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<partinfo>BBa_K4960015 parameters</partinfo>
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[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.<br>
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[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.

Revision as of 20:46, 10 October 2023

pvc16

Properties

PVC16 is a part of PVC Sheath Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.
[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.