Difference between revisions of "Part:BBa K4604023:Design"
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In order to protect our system from high toxin concentrations it is designed to constitutively produce an antitoxin (MazE) to neutralize the toxin (MazF) during the initial stage of AdoCbl production. Once produced, AdoCbl inhibits the toxin expression through binding to the negatively regulating riboswitch, leading to cell survival. In the absence of sufficient AdoCbl production, toxin levels rise due to an inactive riboswitch structure, inducing cell death. | In order to protect our system from high toxin concentrations it is designed to constitutively produce an antitoxin (MazE) to neutralize the toxin (MazF) during the initial stage of AdoCbl production. Once produced, AdoCbl inhibits the toxin expression through binding to the negatively regulating riboswitch, leading to cell survival. In the absence of sufficient AdoCbl production, toxin levels rise due to an inactive riboswitch structure, inducing cell death. | ||
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Latest revision as of 20:18, 10 October 2023
piG_21 (tetR_bluB_riboK12_mazF_AmpProm_mazE)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 710
Illegal BamHI site found at 2756 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1603
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2238
Illegal BsaI site found at 2484
Illegal BsaI site found at 3045
Design Notes
In order to protect our system from high toxin concentrations it is designed to constitutively produce an antitoxin (MazE) to neutralize the toxin (MazF) during the initial stage of AdoCbl production. Once produced, AdoCbl inhibits the toxin expression through binding to the negatively regulating riboswitch, leading to cell survival. In the absence of sufficient AdoCbl production, toxin levels rise due to an inactive riboswitch structure, inducing cell death.