Difference between revisions of "Part:BBa K4724083"
Line 10: Line 10:
  
 
<html>
 
<html>
−
<img src="https://static.igem.wiki/teams/4724/wiki/engineer8.jpg">
+
<img src="https://static.igem.wiki/teams/4724/wiki/engineer8.jpg" style="height:40vh;">
 
</html>
 
</html>
  

Revision as of 19:49, 10 October 2023


DsbA-LSPETase

Insert the signal peptide DsbA gene sequence into the vector pET-22b (+) - LSPET-12, and construct a recombinant plasmid using Gibson assembly method to promote the soluble expression of the original target fragment LSPET protein.

Characterize the results


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal NgoMIV site found at 442
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]