Difference between revisions of "Part:BBa K4724083:Design"
Yaoyaowuqi (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
− | + | The DNA sequences of LSPETase and TrxA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of the TrxA tag at the N-terminus of LSPETase. | |
===Source=== | ===Source=== |
Revision as of 19:38, 10 October 2023
DsbA-LSPETase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1186
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
Illegal NgoMIV site found at 442
Illegal AgeI site found at 529 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DNA sequences of LSPETase and TrxA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of the TrxA tag at the N-terminus of LSPETase.
Source
An enzyme identified in the laboratory of our research group that can identify PET.