Difference between revisions of "Part:BBa K4724083:Design"

 
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===Design Notes===
 
===Design Notes===
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The DsbA gene sequence was inserted into the vector pET-22b (+) - LSPET-12 and a recombinant plasmid was constructed using the Gibson assembly method.
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The DNA sequences of LSPETase and TrxA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of the TrxA tag at the N-terminus of LSPETase.
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===Source===
 
===Source===

Revision as of 19:38, 10 October 2023


DsbA-LSPETase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal NgoMIV site found at 442
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequences of LSPETase and TrxA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of the TrxA tag at the N-terminus of LSPETase.

Source

An enzyme identified in the laboratory of our research group that can identify PET.

References