Difference between revisions of "Part:BBa K4724075:Design"
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
 
 
<html>
 
<html>
 
<h2 style="font-weight:600">NusA-LSPET </h2>
 
<h2 style="font-weight:600">NusA-LSPET </h2>
 
</html>
 
</html>
 +
 +
<partinfo>BBa_K4724075 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 19:23, 10 October 2023


NusA-LSPET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal XhoI site found at 2344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1600
    Illegal AgeI site found at 1687
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA of LSPETase and NusA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of NusA at the N-terminus of LSPETase.

Source

Laboratory design in our research group.

References