Difference between revisions of "Part:BBa K4724072"
 
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Disulfide bond formation protein A (DsbA) is an oxidoreductase enzyme present in the periplasmic space of Escherichia coli, which catalyzes the formation of disulfide bonds during the folding process of nascent proteins. It can transfer the produced proteins to the periplasmic space and catalyze the formation of disulfide bonds in nascent proteins to promote their soluble expression. The accumulation of proteins expressed in the periplasmic space can be utilized for protein purification and prevents interactions with cytoplasmic components.
 
Disulfide bond formation protein A (DsbA) is an oxidoreductase enzyme present in the periplasmic space of Escherichia coli, which catalyzes the formation of disulfide bonds during the folding process of nascent proteins. It can transfer the produced proteins to the periplasmic space and catalyze the formation of disulfide bonds in nascent proteins to promote their soluble expression. The accumulation of proteins expressed in the periplasmic space can be utilized for protein purification and prevents interactions with cytoplasmic components.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4724072 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4724070 parameters</partinfo>
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Latest revision as of 19:21, 10 October 2023

DsbA

Disulfide bond formation protein A (DsbA) is an oxidoreductase enzyme present in the periplasmic space of Escherichia coli, which catalyzes the formation of disulfide bonds during the folding process of nascent proteins. It can transfer the produced proteins to the periplasmic space and catalyze the formation of disulfide bonds in nascent proteins to promote their soluble expression. The accumulation of proteins expressed in the periplasmic space can be utilized for protein purification and prevents interactions with cytoplasmic components.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]