Difference between revisions of "Part:BBa K200015"
| Line 1: | Line 1: | ||
==DpnII== | ==DpnII== | ||
| − | This coding region is to be used in the composite part BBa_K200009 for the genomic deletion module of [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] by Imperial College's 2009 iGEM team. | + | This coding region is to be used in the composite part [https://parts.igem.org/Part:BBa_K200009 BBa_K200009] for the genomic deletion module of [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] by Imperial College's 2009 iGEM team. |
| + | |||
| + | ===Detail=== | ||
| + | This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. | ||
| + | The working temperature is 37°C. | ||
| + | |||
| + | |||
| + | DpnII is a 4 base cutter, leaving a 4 base overhang. | ||
| + | |||
| + | <br> | ||
| + | The cutting site is as shown: | ||
| + | <br><br> | ||
| + | [[Image:II09_DpnII.png]] | ||
| + | <br><br> | ||
| + | |||
| + | ===Usage and Biology=== | ||
| + | |||
| + | Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs | ||
| + | |||
| + | |||
| + | The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project as part of the genome deletion module. | ||
Latest revision as of 21:39, 19 October 2009
DpnII
This coding region is to be used in the composite part BBa_K200009 for the genomic deletion module of [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] by Imperial College's 2009 iGEM team.
Detail
This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37°C.
DpnII is a 4 base cutter, leaving a 4 base overhang.
The cutting site is as shown:
Usage and Biology
Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs
The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module.