Difference between revisions of "Part:BBa K200015"

(New page: DpnII This coding region is to be used in the composite part BBa_K200009 for the genomic deletion module of The E.ncapsulator by Im...)
 
 
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DpnII
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==DpnII==
  
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This coding region is to be used in the composite part BBa_K200009 for the genomic deletion module of [[http://2009.igem.org/Team:Imperial_College_London |The E.ncapsulator]] by Imperial College's 2009 iGEM team.
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This coding region is to be used in the composite part [https://parts.igem.org/Part:BBa_K200009 BBa_K200009] for the genomic deletion module of [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] by Imperial College's 2009 iGEM team.
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===Detail===
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This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.'  Its activity is blocked by dam methylation.
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The working temperature is 37°C.
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DpnII is a 4 base cutter, leaving a 4 base overhang.
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<br>
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The cutting site is as shown:
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<br><br>
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[[Image:II09_DpnII.png]] 
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<br><br>
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===Usage and Biology===
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Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs
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The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project as part of the genome deletion module.

Latest revision as of 21:39, 19 October 2009

DpnII

This coding region is to be used in the composite part BBa_K200009 for the genomic deletion module of [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] by Imperial College's 2009 iGEM team.

Detail

This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37°C.


DpnII is a 4 base cutter, leaving a 4 base overhang.


The cutting site is as shown:

II09 DpnII.png

Usage and Biology

Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs


The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module.