Difference between revisions of "Part:BBa K200009"
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<partinfo>BBa_K200009 short</partinfo> | <partinfo>BBa_K200009 short</partinfo> | ||
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| − | This | + | This part encodes a ribosome binding site and DpnII coding region (from [https://parts.igem.org/Part:BBa_K200015 BBa_K200015]).<br> |
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===Detail=== | ===Detail=== | ||
Latest revision as of 21:38, 19 October 2009
DpnII Composite
This part encodes a ribosome binding site and DpnII coding region (from BBa_K200015).
Detail
This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37°C.
DpnII is a 4 base cutter, leaving a 4 base overhang.
The cutting site is as shown:
Usage and Biology
Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs
The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[http://www.neb.com/nebecomm/products/productR0543.asp Information from NEB]