Difference between revisions of "Part:BBa K4593007:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4593007 short</partinfo> | ||
+ | <partinfo>BBa_K4593007 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | We added biotin at the 3’ end to test the affinity of the aptamer | ||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | In 2016, Regina Stoltenburg and her team discovered PA#2/8 is an aptamer binding the S.aureus protein A. We employed a computational approach using the trained BERT language model in a dry lab setting. Through this screening of large amount of aptamer mutants, we discovered this aptamer as effective. | ||
+ | |||
+ | ===References=== | ||
+ | [1] Stoltenburg, R., Krafčiková, P., Víglaský, V., & Strehlitz, B. (2016). G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports, 6(1). https://doi.org/10.1038/srep33812 |
Latest revision as of 16:28, 10 October 2023
PA#2/8-B
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added biotin at the 3’ end to test the affinity of the aptamer
Source
In 2016, Regina Stoltenburg and her team discovered PA#2/8 is an aptamer binding the S.aureus protein A. We employed a computational approach using the trained BERT language model in a dry lab setting. Through this screening of large amount of aptamer mutants, we discovered this aptamer as effective.
References
[1] Stoltenburg, R., Krafčiková, P., Víglaský, V., & Strehlitz, B. (2016). G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports, 6(1). https://doi.org/10.1038/srep33812