Difference between revisions of "Part:BBa K175001"
(New page: __NOTOC__ <partinfo>BBa_K175001 short</partinfo> This part encodes for trbK, a small 75 amino acid cytoplasmic membrane lipoprotein which handles the entry exclusion in the R751 plasmid. ...) |
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<partinfo>BBa_K175001 short</partinfo> | <partinfo>BBa_K175001 short</partinfo> | ||
| − | This part encodes for trbK, a small 75 amino acid cytoplasmic membrane lipoprotein which handles the entry exclusion in the R751 plasmid. Expression of this part in a cell without R751 will | + | This part encodes for trbK, a small 75 amino acid cytoplasmic membrane lipoprotein which handles the entry exclusion in the R751 plasmid. Expression of this part in a cell without R751 will block incoming transfers from R751 donors. This is designed to prevent redundant transfers from occurring. Note that the presence of entry exclusion has been linked to the prevention of lethal zygosis. |
If the entry exclusion (Eex) index is defined as the transfer frequency for receiver cells not containing the trbK protein divided by the transfer frequency for receiver cells containing the trbK protein than for R751 Eex index = 44,000 (See Table 5 of Haase, J., Kalkum, M. & Lanka, E. TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP alpha plasmid RP4. J. Bacteriol. 178, 6720–6729 (1996).[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178567]) | If the entry exclusion (Eex) index is defined as the transfer frequency for receiver cells not containing the trbK protein divided by the transfer frequency for receiver cells containing the trbK protein than for R751 Eex index = 44,000 (See Table 5 of Haase, J., Kalkum, M. & Lanka, E. TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP alpha plasmid RP4. J. Bacteriol. 178, 6720–6729 (1996).[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178567]) | ||
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| + | See [[Part:BBa_K175001:Experience | Experience]] page for more information. | ||
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Latest revision as of 21:24, 19 October 2009
trbK (entry exclusion) of IncP beta R751 plasmid
This part encodes for trbK, a small 75 amino acid cytoplasmic membrane lipoprotein which handles the entry exclusion in the R751 plasmid. Expression of this part in a cell without R751 will block incoming transfers from R751 donors. This is designed to prevent redundant transfers from occurring. Note that the presence of entry exclusion has been linked to the prevention of lethal zygosis.
If the entry exclusion (Eex) index is defined as the transfer frequency for receiver cells not containing the trbK protein divided by the transfer frequency for receiver cells containing the trbK protein than for R751 Eex index = 44,000 (See Table 5 of Haase, J., Kalkum, M. & Lanka, E. TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP alpha plasmid RP4. J. Bacteriol. 178, 6720–6729 (1996).[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178567])
See Experience page for more information.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]