Difference between revisions of "Part:BBa K4830000:Design"

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===Design Notes===
 
===Design Notes===
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No considerations required to use this sequence design.
+
The design of RNF2 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
  
 
===Source===
 
===Source===
  
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Search and replace paper
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The original RNF2 pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of and TWIST Bioscience we synthesized those and other necessary sequences, which were further combined together with 65777 backbone in our lab.
  
 
===References===
 
===References===
 +
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Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.

Latest revision as of 16:20, 10 October 2023


RNF pegRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of RNF2 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.

Source

The original RNF2 pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of and TWIST Bioscience we synthesized those and other necessary sequences, which were further combined together with 65777 backbone in our lab.

References

Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.