Difference between revisions of "Part:BBa K4960023"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4960023</partinfo><br>
 
<partinfo>BBa_K4960023</partinfo><br>
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This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows:
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This part is responsible for payloads loading Cre.
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5' - ATAACTTCGTATA - GCATACAT - TATACGAAGTTAT - 3'
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3' - TATTGAAGCATAT - CGTATGTA - ATATGCTTCAATA - 5'
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HiBiT is a polypeptide tag, can be linked to any protein.
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This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4960023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960023 SequenceAndFeatures</partinfo>
 +
This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows:
 +
5' - ATAACTTCGTATA - GCATACAT - TATACGAAGTTAT - 3'
 +
3' - TATTGAAGCATAT - CGTATGTA - ATATGCTTCAATA - 5'
 +
HiBiT is a polypeptide tag, can be linked to any protein.
 +
This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.
  
  

Revision as of 15:27, 10 October 2023


BBa_K4960023
This part is responsible for payloads loading Cre.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 599
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 285
  • 1000
    COMPATIBLE WITH RFC[1000]

This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows: 5' - ATAACTTCGTATA - GCATACAT - TATACGAAGTTAT - 3' 3' - TATTGAAGCATAT - CGTATGTA - ATATGCTTCAATA - 5' HiBiT is a polypeptide tag, can be linked to any protein. This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.