Difference between revisions of "Part:BBa K4960023"

Revision as of 15:22, 10 October 2023

BBa_K4960023
This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows: 5' - ATAACTTCGTATA - GCATACAT - TATACGAAGTTAT - 3' 3' - TATTGAAGCATAT - CGTATGTA - ATATGCTTCAATA - 5' HiBiT is a polypeptide tag, can be linked to any protein. This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 599
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 285
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K4960023This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows:
+<partinfo>BBa_K4960023</partinfo><br>
+This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows:
 ' - ATAACTTCGTATA - GCATACAT - TATACGAAGTTAT - 3'
 ' - TATTGAAGCATAT - CGTATGTA - ATATGCTTCAATA - 5'
 HiBiT is a polypeptide tag, can be linked to any protein.
-This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.</partinfo>
+This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.