Difference between revisions of "Part:BBa K200004"

Latest revision as of 21:18, 19 October 2009

TaqI

This sequence codes for the restriction enzyme TaqI. It will be used in the composite part BBa_K200010 by Imperial iGEM 2009 in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project.

This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation.

The working temperature is 65°C.

As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 18
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 157

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K200004 short</partinfo>
-The methylase encoded by the dam gene (Dam methylase) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.
+This sequence codes for the restriction enzyme TaqI. It will be used in the composite part [https://parts.igem.org/wiki/index.php/Part:BBa_K200010 BBa_K200010] by Imperial iGEM 2009 in <i>[http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator]</i> project.
+This is a Type II restriction enzyme that recognises the sequence TCGA.  Its activity can be blocked by dam methylation. <br>
+The working temperature is 65°C. <br>
+As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.
 <!-- Add more about the biology of this part here