Difference between revisions of "Part:BBa K4743032"
 
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We use the gene HPD and LAD to let B.Subtilis to produce HMB and KIC
 
We use the gene HPD and LAD to let B.Subtilis to produce HMB and KIC
  
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<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/hmb-production-pathway.png" </html>
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<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/hmb-production-pathway.png" </html>
  
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<center>Figure 1. The Matabolic pathway of HMB and KIC production.</center>
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<center>Figure 1. The Matabolic pathway and map of HMB and KIC production.</center>
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<span class='h3bb'><big>'''Data of HMB production'''</big></span>
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After estimation, we've got 3.97g/L production of HMB
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<html><img style="width:600px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/hmb-hplc-peak-igem-upload.png" </html>
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<center>Figure 2. The quantitative analysis for HMB.</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:15, 10 October 2023


Bacillus subtilis HMB production set

This part is uesd for the production of HMB and KIC in Bacillus subtilis

Overall metabolic pathway of HMB

We use the gene HPD and LAD to let B.Subtilis to produce HMB and KIC

Figure 1. The Matabolic pathway and map of HMB and KIC production.

Data of HMB production

After estimation, we've got 3.97g/L production of HMB

Figure 2. The quantitative analysis for HMB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 511
    Illegal XhoI site found at 193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2162
  • 1000
    COMPATIBLE WITH RFC[1000]