Difference between revisions of "Part:BBa K4830000:Design"

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===Design Notes===
 
===Design Notes===
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No considerations required to use this sequence design.
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The design of RNF2 pegRNA included the following considerations. CACC overhang before the spacer, rttPBS length, the A to G edit, and polyA tail.
  
 
===Source===
 
===Source===

Revision as of 14:49, 10 October 2023


RNF pegRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of RNF2 pegRNA included the following considerations. CACC overhang before the spacer, rttPBS length, the A to G edit, and polyA tail.

Source

The original RNF2 pegRNA sequence containing spacer and rttpbs was reported in Prime Editor 2 paper cited in References. We used those sequences and with the help of and TWIST Bioscience synthesized the parts, which were further pieced together with 65777 backbone in our lab.

References

Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.