Difference between revisions of "Part:BBa K4604006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly. | + | Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly. |
| − | + | ||
| − | + | ||
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===Source=== | ===Source=== | ||
Revision as of 12:30, 10 October 2023
bluB mutated
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly.
Source
Taken from Mycobacterium Smegmatis, synthesized by IDT.