Difference between revisions of "Part:BBa K4605011"

 
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<partinfo>BBa_K4605011 short</partinfo>
 
<partinfo>BBa_K4605011 short</partinfo>
  
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C. glutamicum has the native pcpS gene, which expresses PPTase(4'-phosphopantetheinyl transferase). The PPTase is of great significance because it converts the apo-form of the BpsA into its active holo-form by attaching coenzyme A to the peptide carrier domain (PCP).However,in K.xylinus,there is no pcpS exist.Thus,we need to build a bpsA-pcpS fragment so that bpsA can be activated to catalyze the reaction.
  
 
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Revision as of 11:43, 10 October 2023


Expressing PPTase to activate bpsA

C. glutamicum has the native pcpS gene, which expresses PPTase(4'-phosphopantetheinyl transferase). The PPTase is of great significance because it converts the apo-form of the BpsA into its active holo-form by attaching coenzyme A to the peptide carrier domain (PCP).However,in K.xylinus,there is no pcpS exist.Thus,we need to build a bpsA-pcpS fragment so that bpsA can be activated to catalyze the reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
    Illegal NgoMIV site found at 156
    Illegal NgoMIV site found at 261
    Illegal NgoMIV site found at 294
    Illegal AgeI site found at 285
  • 1000
    COMPATIBLE WITH RFC[1000]