Difference between revisions of "Part:BBa K4692000:Design"

 
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===Design Notes===
 
===Design Notes===
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The DNA sequence has been optimized for expression in the yeast Pichia pastoris.
+
The DNA sequence has been optimized for expression in the yeast Saccharomyces cerevisiae. The native secretion signal has been replaced with the alpha mating factor sequence from Saccharomyces cerevisiae. A GS linker has been added between the alpha mating factor sequence and the coding sequence.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
This gene is from the filamenteous fungus Aspergillus japonicus BCC18313 (Genbank accession EU786166.1).
+
This gene is from Escherichia coli MG1655 (EcoCyc accession EG10033).
  
 
===References===
 
===References===
 +
https://ecocyc.org/gene?orgid=ECOLI&id=GLUCOSE-1-PHOSPHAT-MONOMER

Latest revision as of 11:14, 10 October 2023


Phytase from Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence has been optimized for expression in the yeast Saccharomyces cerevisiae. The native secretion signal has been replaced with the alpha mating factor sequence from Saccharomyces cerevisiae. A GS linker has been added between the alpha mating factor sequence and the coding sequence.

Source

This gene is from Escherichia coli MG1655 (EcoCyc accession EG10033).

References

https://ecocyc.org/gene?orgid=ECOLI&id=GLUCOSE-1-PHOSPHAT-MONOMER