Difference between revisions of "Part:BBa K243003"
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[[Image:Dig2b.JPG|overview of immunohistochemical applications]]<br> | [[Image:Dig2b.JPG|overview of immunohistochemical applications]]<br> | ||
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| − | We used this part to connect our universal restriction endonuclease protein domains to digoxigenin labeled oligos. These oligos were hybridized with ssDNA and so the made a connection between the protein and the DNA. | + | We used this part to connect our universal restriction endonuclease protein domains to digoxigenin labeled oligos. These oligos were hybridized with ssDNA and so the made a connection between the protein and the DNA. Another possiblity is to use TFO's(triplex-forming oligonucleotides)labeled with digoxigenin, to made a connection between dsDNA and protein. |
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Revision as of 17:17, 19 October 2009
Digoxigenin binding protein (DigA)
This part functions as a linker to another part/protein ,which is labeled with digoxigenin.
Usage and Biology
Normally a digoxigenin antibody is use for Nonradioactive in situ hybridization.
We used this part to connect our universal restriction endonuclease protein domains to digoxigenin labeled oligos. These oligos were hybridized with ssDNA and so the made a connection between the protein and the DNA. Another possiblity is to use TFO's(triplex-forming oligonucleotides)labeled with digoxigenin, to made a connection between dsDNA and protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]