Difference between revisions of "Part:BBa K185034"
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<partinfo>BBa_K185034 short</partinfo> | <partinfo>BBa_K185034 short</partinfo> | ||
− | This part is based on QPI(Q04510), which made by early team. We add a promoter(J23116) on the begining of QPI and add RBS(B0030), RelB(K185048) gene and Double-terminator(B0015) at the end of it in order to express RelB protein. Because of QPI | + | This part is based on QPI(Q04510), which made by early team. We add a promoter(J23116) on the begining of QPI and add RBS(B0030), RelB(K185048) gene and Double-terminator(B0015) at the end of it in order to express RelB protein. Because of QPI containing CI gene so that this device can be induced by Tryptophan. |
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Latest revision as of 17:05, 19 October 2009
Promoter J23116+Q04510+B0034+RelB+Double-terminator
This part is based on QPI(Q04510), which made by early team. We add a promoter(J23116) on the begining of QPI and add RBS(B0030), RelB(K185048) gene and Double-terminator(B0015) at the end of it in order to express RelB protein. Because of QPI containing CI gene so that this device can be induced by Tryptophan.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1159
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]