Difference between revisions of "Part:BBa K4759201"
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Since the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB
 
Since the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB
  
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===Design Notes===
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===Design ===
 
codon-optimized
 
codon-optimized
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4759201 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4759201 SequenceAndFeatures</partinfo>
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the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB
  
 
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Revision as of 06:56, 10 October 2023


T7-RBS1-camA-RBS2-camB

Since the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB

Design

codon-optimized Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1366
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1366
    Illegal BamHI site found at 1360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1366
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1366
    Illegal AgeI site found at 124
    Illegal AgeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]

the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB