Difference between revisions of "Part:BBa K4711054"

 
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=Usage and Biology=
 
=Usage and Biology=
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The mCYC promoter can be bound by the DNA binding domain of LexA to initiate the expression of downstream upo1 benzo[a]pyrene-degrading enzymes.
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  <figure>
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      <img src="https://static.igem.wiki/teams/4711/wiki/engineering/7-2.png"width="100%" style="float:center">
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      <figcaption>
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      <p style="font-size:1rem">
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Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG
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      </figcaption>
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  </figure>
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===Source===
 
===Source===

Latest revision as of 06:50, 10 October 2023


mCYC+Upo1

Usage and Biology

The mCYC promoter can be bound by the DNA binding domain of LexA to initiate the expression of downstream upo1 benzo[a]pyrene-degrading enzymes.

Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG

Source

Potential applications

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1075
    Illegal PstI site found at 941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1075
    Illegal PstI site found at 941
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 675
    Illegal XhoI site found at 149
    Illegal XhoI site found at 1168
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1075
    Illegal PstI site found at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1075
    Illegal PstI site found at 941
    Illegal NgoMIV site found at 629
    Illegal NgoMIV site found at 1379
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 788
    Illegal BsaI.rc site found at 1097