Difference between revisions of "Part:BBa K4763007:Design"
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===Design Notes===
 
===Design Notes===
−
 
+
Optimized sequence to disrupt the internal NdeI, NcoI and NsiI sites for convenience.
  
  
 
===Source===
 
===Source===
−
Sequence was obtained from NCBI (NM_123774.4). Optimized to disrupt the internal NdeI, NcoI and NsiI sites for convenience.
+
Sequence was obtained from NCBI (NM_123774.4).  
  
 
===References===
 
===References===

Revision as of 06:49, 10 October 2023


AtPCS (phytochelatin synthase) coding sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 649
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 649
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized sequence to disrupt the internal NdeI, NcoI and NsiI sites for convenience.


Source

Sequence was obtained from NCBI (NM_123774.4).

References