Difference between revisions of "Part:BBa K216011:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K216011=== | ===Applications of BBa_K216011=== | ||
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| + | This part is designed to produce a visible response (EYFP) when induced by the signal transduction cascade controlled by the hybrid Trz signal transduction protein; see notes on part BBa_K216004 for details of how this works. | ||
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| + | [[Image:PompC-EYFP.jpg ]] | ||
===User Reviews=== | ===User Reviews=== | ||
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| + | '''Initial tests:''' (Edinburgh iGEM 2009) As you can see from the photo above, this construct definitely produces EYFP and shows strong fluorescence. We have not yet performed experiments to confirm its inducibility with EnvZ agonist procaine (though the equivalent GFP construct, used to characterise the ''ompC'' promoter, showed inducibility) or via the hybrid signal transduction pathway, as our permit to work with explosives has not yet come through. | ||
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Latest revision as of 15:45, 19 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K216011
This part is designed to produce a visible response (EYFP) when induced by the signal transduction cascade controlled by the hybrid Trz signal transduction protein; see notes on part BBa_K216004 for details of how this works.
User Reviews
Initial tests: (Edinburgh iGEM 2009) As you can see from the photo above, this construct definitely produces EYFP and shows strong fluorescence. We have not yet performed experiments to confirm its inducibility with EnvZ agonist procaine (though the equivalent GFP construct, used to characterise the ompC promoter, showed inducibility) or via the hybrid signal transduction pathway, as our permit to work with explosives has not yet come through.
UNIQ14ffe75efa4e8548-partinfo-00000000-QINU UNIQ14ffe75efa4e8548-partinfo-00000001-QINU