Difference between revisions of "Part:BBa K4156083:Design"
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===Design Notes===
 
===Design Notes===
  
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It is important to note that φ174E is a new part that we uploaded this year. And as a toxic protein, it does not exist in the igem whitelist. We have drafted a safety precaution about φ174E and submitted the check-in form to igem official. The details can be seen on our wiki.
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It is important to note that φ174E does not exist in the igem whitelist. We have drafted a safety precaution about φ174E and submitted the check-in form to igem official. The details can be seen on our wiki.
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=Demonstration of the function of the lysis gene=
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'''Group''': [https://2023.igem.wiki/ouc-china/parts iGEM Team OUC-China 2023]
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'''Author''': Qingyu Wu
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'''Summary''': Successfully demonstrated the lysis function of this gene
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After induction with IPTG for 2-3 hours, the lysin protein can almost completely kill all bacteria. According to the results of SDS-PAGE in the figure below, it can be observed that there are almost no protein bands in the induced group. This is because a large amount of protease is released after cell death, leading to protein degradation, indicating that the lysin protein is functioning.
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<html>
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  <figure>
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      <img src="https://static.igem.wiki/teams/4711/wiki/results/figure-13.png"width="100%" style="float:center">
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      <figcaption>
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      <p style="font-size:1rem">
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Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG
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      </figcaption>
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  </figure>
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</html>
  
 
===Source===
 
===Source===

Latest revision as of 05:26, 10 October 2023


φ174E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is important to note that φ174E does not exist in the igem whitelist. We have drafted a safety precaution about φ174E and submitted the check-in form to igem official. The details can be seen on our wiki.

Demonstration of the function of the lysis gene

Group: iGEM Team OUC-China 2023

Author: Qingyu Wu

Summary: Successfully demonstrated the lysis function of this gene

After induction with IPTG for 2-3 hours, the lysin protein can almost completely kill all bacteria. According to the results of SDS-PAGE in the figure below, it can be observed that there are almost no protein bands in the induced group. This is because a large amount of protease is released after cell death, leading to protein degradation, indicating that the lysin protein is functioning.

Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG

Source

φ174E is a lysis gene from phage Φx174.

References