Difference between revisions of "Part:BBa K4765110"

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| '''Figure1 Sequencing map of LCA'''
 
| '''Figure1 Sequencing map of LCA'''
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Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3.
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Sequencing iss performed using the primer：Kan-F: 5-ATTCTCACCGGATTCAGT-3.
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Revision as of 04:40, 10 October 2023

Twister P1 + T7_RBS + intimin-LCA fusion + stem-loop

contributed by Fudan iGEM 2023

Introduction

We’ve developed an E. coli-cyanobacteria adhesion module by transfecting intimin-LCA fusion. Intimin-LCA fusion is composed of intimin and LCA. is a lectin from Lentils that can recognize α-linked mannose residues. It is a common lectin that can specifically bind to the LPS on the surface of S. elongatus PCC7942. Intimin includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[1] , possesses the outer membrane anchoring of LCA. We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP.

Usage and Biology

This biological component delivers LCA to the surface of E. coli, facilitating adhesion between E. coli, and S. elongatus PCC7942. We envision that the adhesion between cyanobacteria and E. coli can promote the exchange of substances within the biofilm, enhancing the biofilm's survivability.

Characterization

Sequencing map

contributed by Fudan iGEM 2023
Figure1 Sequencing map of LCA

Sequencing iss performed using the primer：Kan-F: 5-ATTCTCACCGGATTCAGT-3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305


Reference

  1. ↑ Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins. ACS Synthetic Biology, 4(4), 463–473. https://doi.org/10.1021/sb500252a