Difference between revisions of "Part:BBa K4759001"

(Jiangnan-China 2023)
Line 3: Line 3:
 
<partinfo>BBa_K4759001 short</partinfo>
 
<partinfo>BBa_K4759001 short</partinfo>
  
a gene coding for multifunctional bacterial P450 monooxygenase CYP107D1(Olep), which is from the oleandomycin synthesis pathway of Streptomyces antibioticus. Cytochrome P450s (P450s) are heme-containing enzymes that catalyze various types of chemical reactions on a variety of substrates, The bacterial P450 OleP,  is an epoxidase involved in the tailoring steps of the biosynthetic pathway of the antibiotic oleandomycin, OleP not only can catalyzes a regio- and stereospecific reaction of epoxidation at the C8-C8a of the macrolactone ring, but also can Regio- and stereoselective hydroxylation of bile acid such as lithocholic acid (LCA), the production can be served as potential drug candidates to treat symptoms associated with the coronavirus disease (COVID-19) infection. In our project, we found that Olep also can Regio- and stereoselective hydroxylation of deoxycholic acid (DCA) to 6β-DCA. P450s are thought to be reliable, effective, and ecofriendly biocatalysts for the synthesis of valuable compounds in recombinant hosts.
+
a gene coding for multifunctional bacterial P450 monooxygenase CYP107D1(Olep), which is from the oleandomycin synthesis pathway of Streptomyces antibioticus. Cytochrome P450s (P450s) are heme-containing enzymes that catalyze various types of chemical reactions on a variety of substrates, The bacterial P450 Olep,  is an epoxidase involved in the tailoring steps of the biosynthetic pathway of the antibiotic oleandomycin, OleP not only can catalyzes a regio- and stereospecific reaction of epoxidation at the C8-C8a of the macrolactone ring, but also can Regio- and stereoselective hydroxylation of bile acid such as lithocholic acid (LCA), the production can be served as potential drug candidates to treat symptoms associated with the coronavirus disease (COVID-19) infection. In our project, we found that Olep also can Regio- and stereoselective hydroxylation of deoxycholic acid (DCA) to 6β-DCA. P450s are thought to be reliable, effective, and ecofriendly biocatalysts for the synthesis of valuable compounds in recombinant hosts.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:38, 10 October 2023


OleP

a gene coding for multifunctional bacterial P450 monooxygenase CYP107D1(Olep), which is from the oleandomycin synthesis pathway of Streptomyces antibioticus. Cytochrome P450s (P450s) are heme-containing enzymes that catalyze various types of chemical reactions on a variety of substrates, The bacterial P450 Olep, is an epoxidase involved in the tailoring steps of the biosynthetic pathway of the antibiotic oleandomycin, OleP not only can catalyzes a regio- and stereospecific reaction of epoxidation at the C8-C8a of the macrolactone ring, but also can Regio- and stereoselective hydroxylation of bile acid such as lithocholic acid (LCA), the production can be served as potential drug candidates to treat symptoms associated with the coronavirus disease (COVID-19) infection. In our project, we found that Olep also can Regio- and stereoselective hydroxylation of deoxycholic acid (DCA) to 6β-DCA. P450s are thought to be reliable, effective, and ecofriendly biocatalysts for the synthesis of valuable compounds in recombinant hosts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 16
    Illegal AgeI site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]