Difference between revisions of "Part:BBa K5023013"

Revision as of 01:28, 10 October 2023

PAR_Ble_SP7_FAST-PETase_mCherry_Ter

This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, gene reporter mCherry, and the terminator. Designed to express and secrete Fast-PETase with verification by mCherry reporter in Chlamydomonas reinhardtii, utilizing pJP32 backbone.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 698
Illegal PstI site found at 2111
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 249
Illegal NheI site found at 1211
Illegal PstI site found at 698
Illegal PstI site found at 2111
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1616
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 698
Illegal PstI site found at 2111
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 698
Illegal PstI site found at 2111
Illegal NgoMIV site found at 1079
Illegal NgoMIV site found at 1140
Illegal NgoMIV site found at 1420
Illegal NgoMIV site found at 3174
1000
COMPATIBLE WITH RFC[1000]

Revision as of 21:17, 8 October 2023 (view source) Registry (Talk \| contribs)		Revision as of 01:28, 10 October 2023 (view source) Lucasovelar (Talk \| contribs) Newer edit →
Line 3:		Line 3:
	<partinfo>BBa_K5023013 short</partinfo>		<partinfo>BBa_K5023013 short</partinfo>

−	~~With the~~ PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, gene reporter mCherry, and the terminator. Designed to express and secrete Fast-PETase with verification by mCherry reporter in Chlamydomonas reinhardtii, utilizing pJP32 backbone.	+	This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, gene reporter mCherry, and the terminator. Designed to express and secrete Fast-PETase with verification by mCherry reporter in Chlamydomonas reinhardtii, utilizing pJP32 backbone.
		+