Difference between revisions of "Part:BBa K4759001"

Revision as of 00:58, 10 October 2023

OleP

a gene coding for multifunctional bacterial P450 monooxygenase CYP107D1(Olep), which is from the oleandomycin synthesis pathway of Streptomyces antibioticus. Cytochrome P450s (P450s) are heme-containing enzymes that catalyze various types of chemical reactions on a variety of substrates, The bacterial P450 OleP, is an epoxidase involved in the tailoring steps of the biosynthetic pathway of the antibiotic oleandomycin, OleP not only can catalyzes a regio- and stereospecific reaction of epoxidation at the C8-C8a of the macrolactone ring, but also can Regio- and stereoselective hydroxylation of bile acid such as lithocholic acid (LCA), the production can be served as potential drug candidates to treat symptoms associated with the coronavirus disease (COVID-19) infection. In our project, we found that Olep also can Regio- and stereoselective hydroxylation of deoxycholic acid (DCA) to 6β-DCA. P450s are thought to be reliable, effective, and ecofriendly biocatalysts for the synthesis of valuable compounds in recombinant hosts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 16
Illegal AgeI site found at 114
1000
COMPATIBLE WITH RFC[1000]

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 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+OleP, the cytochrome P450 epoxidase from Streptomyces antibioticus involved in Oleandomycin biosynthesis: functional analysis and crystallographic structure in complex with clotrimazole.
+PDB DOI:  https://doi.org/10.2210/pdb4XE3/pdb
+Classification: OXIDOREDUCTASE
+Organism(s): Streptomyces antibioticus
+Expression System: Escherichia coli BL21(DE3)
+https://static.igem.wiki/teams/4759/wiki/4xe3.png
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