Difference between revisions of "Part:BBa K4897014"

Latest revision as of 00:17, 10 October 2023

LUXI promoter and EL222 binding sequence

What is it?

This part allows the gene expression during a dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter.

Usage and Biology

With this part built into our blue light suicide system, once blue light is undetectable by EL222, which means a dark environment is provided at night, the formation of EL222 dimers is terminated and the RNAP is allowed to bind back to the -35 to -10 region of the luxI promoter. This thereby activates the expression of the two types of CAS9 (CAS9-A and CAS9-R) and their sgRNA sequence (sgRNA-A and sgRNA-R). This allows our target bacteria to be killed and ensures the safety of envioronment.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4897014 short</partinfo>
-This part allows the gene expression during the Dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter.
+===What is it?===
+This part allows the gene expression during a dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 With this part built into our blue light suicide system, once blue light is undetectable by EL222, which means a dark environment is provided at night, the formation of EL222 dimers is terminated and the RNAP is allowed to bind back to the -35 to -10 region of the luxI promoter. This thereby activates the expression of the two types of CAS9 (CAS9-A and CAS9-R) and their sgRNA sequence (sgRNA-A and sgRNA-R). This allows our target bacteria to be killed and ensures the safety of envioronment.