Difference between revisions of "Part:BBa K4897014"
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<partinfo>BBa_K4897014 short</partinfo> | <partinfo>BBa_K4897014 short</partinfo> | ||
| − | + | ===What is it?=== | |
| + | This part allows the gene expression during a dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | With this part built into our blue light suicide system, once blue light is undetectable by EL222, which means a dark environment is provided at night, the formation of EL222 dimers is terminated and the RNAP is allowed to bind back to the -35 to -10 region of the luxI promoter. This thereby activates the expression of the two types of CAS9 (CAS9-A and CAS9-R) and their sgRNA sequence (sgRNA-A and sgRNA-R). This allows our target bacteria to be killed and ensures the safety of envioronment. | ||
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Latest revision as of 00:17, 10 October 2023
LUXI promoter and EL222 binding sequence
What is it?
This part allows the gene expression during a dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter.
Usage and Biology
With this part built into our blue light suicide system, once blue light is undetectable by EL222, which means a dark environment is provided at night, the formation of EL222 dimers is terminated and the RNAP is allowed to bind back to the -35 to -10 region of the luxI promoter. This thereby activates the expression of the two types of CAS9 (CAS9-A and CAS9-R) and their sgRNA sequence (sgRNA-A and sgRNA-R). This allows our target bacteria to be killed and ensures the safety of envioronment.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]