Difference between revisions of "Part:BBa K4895052"
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Haloacid Dehalogenase originating from Delftia Acidovorans, belonging to the α/β hydrolase superfamily. Likely candidate for performing PFAS degradation due to Delftia acidorovans’s ability to subsist solely off of PFOA as a carbon source (Harris, et. al 2022)(10.1016/j.synbio.2022.02.005). | Haloacid Dehalogenase originating from Delftia Acidovorans, belonging to the α/β hydrolase superfamily. Likely candidate for performing PFAS degradation due to Delftia acidorovans’s ability to subsist solely off of PFOA as a carbon source (Harris, et. al 2022)(10.1016/j.synbio.2022.02.005). | ||
Revision as of 23:51, 9 October 2023
DeHa2 (Delftia Acidovorans)
Haloacid Dehalogenase originating from Delftia Acidovorans, belonging to the α/β hydrolase superfamily. Likely candidate for performing PFAS degradation due to Delftia acidorovans’s ability to subsist solely off of PFOA as a carbon source (Harris, et. al 2022)(10.1016/j.synbio.2022.02.005).
The sequence was optimized for the organism E.coli (K12). The GC level was optimized to be Medium (0.33 to 0.66). Existing cutsites were removed and no new ones were created for the following enzymes: BbsI.