Difference between revisions of "Part:BBa K203112"
 
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<partinfo>BBa_K203112 short</partinfo>
 
<partinfo>BBa_K203112 short</partinfo>
  
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A constitutive reference promoter for gene expression and relative promoter characterization in mammalian cells.
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A constitutive reference promoter for gene expression and relative promoter characterization in mammalian cells. Contains a HindIII site between core and proximal promoter for creation of new promoters.
  
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===Usage and Biology===
 
===Usage and Biology===
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Mammalian promoters can be subdivided into several "domains". The core promoter is the binding site of the basal transcription machinery, i.e. RNA polymerase and associated factors. Core promoters differ in composition, but are more or less similar for most genes [1]. The main regulatory domain is the proximal promoter, which is where regulatory elements bind. It can be very large (4kb), meaning that some transcription factors regulate transcription despite being very far away from the RNA polymerase. If the core promoter prevents optimal binding of the basal transcription machinery, promoter strength varies without affecting regulation.
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[1] Heintzman ND, Ren B. The gateway to transcription: identifying, characterizing and understanding promoters in the eukaryotic genome. Cellular and Molecular Life Science 64, 386-400 (2007).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K203112 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K203112 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===

Latest revision as of 13:42, 19 October 2009

JeT; constitutive promoter

A constitutive reference promoter for gene expression and relative promoter characterization in mammalian cells. Contains a HindIII site between core and proximal promoter for creation of new promoters.

Usage and Biology

Mammalian promoters can be subdivided into several "domains". The core promoter is the binding site of the basal transcription machinery, i.e. RNA polymerase and associated factors. Core promoters differ in composition, but are more or less similar for most genes [1]. The main regulatory domain is the proximal promoter, which is where regulatory elements bind. It can be very large (4kb), meaning that some transcription factors regulate transcription despite being very far away from the RNA polymerase. If the core promoter prevents optimal binding of the basal transcription machinery, promoter strength varies without affecting regulation.

[1] Heintzman ND, Ren B. The gateway to transcription: identifying, characterizing and understanding promoters in the eukaryotic genome. Cellular and Molecular Life Science 64, 386-400 (2007).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Has by definition of [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU] an expression strength of 1 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU]. Has by definition of [http://2009.igem.org/Team:Heidelberg/Project_Measurement RMPU] an expression strength of 1 [http://2009.igem.org/Team:Heidelberg/Project_Measurement RMPU].