Difference between revisions of "Part:BBa K4719025"

 
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<partinfo>BBa_K4719025 short</partinfo>
 
<partinfo>BBa_K4719025 short</partinfo>
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==Introduction==
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<b>Vilnius-Lithuania iGEM 2023</b> team's goal was to create <b> synthetic biology tools for <i>in vivo</i> alterations of <i>Komagataeibacter xylinus</i> bacterial cellulose polymer composition</b>. Firstly, we chose to produce a <b>cellulose-chitin copolymer</b> that would later be deacetylated, creating <b>bacterial cellulose-chitosan</b>. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed <b>indigo-dyed cellulose</b> that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of <b>bacterial cellulose and polyhydroxybutyrate (PHB) composite</b>, which is synthesized by <i>K. xylinus</i>.
  
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Vilnius-Lithuania 2023 team performed proteomic analysis of ''K. xylinus'' and identified signal peptide sequence.  
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<hmtl>
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<body>
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<h2> Usage and Biology</h2>
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Proteomic analysis of ''K. xylinus'' was performed and signal peptide sequence was identified.
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<figure>
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<div class = "center" >
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<center><img src = "https://static.igem.wiki/teams/4719/wiki/partai/output-sequence-plot.png" style = "width:400px;"></center>
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</div>
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<figcaption><center><b>Figure 1.</b>SignalIP 6.0 output </center></figcaption>
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</figure>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:23, 9 October 2023


K. xylinus extracellular signal peptide sequence

Introduction

Vilnius-Lithuania iGEM 2023 team's goal was to create synthetic biology tools for in vivo alterations of Komagataeibacter xylinus bacterial cellulose polymer composition. Firstly, we chose to produce a cellulose-chitin copolymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of bacterial cellulose and polyhydroxybutyrate (PHB) composite, which is synthesized by K. xylinus.


<hmtl> <body>

Usage and Biology

Proteomic analysis of K. xylinus was performed and signal peptide sequence was identified. <figure>

<img src = "output-sequence-plot.png" style = "width:400px;">
<figcaption>
Figure 1.SignalIP 6.0 output
</figcaption>

</figure>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]