Difference between revisions of "Part:BBa K233324"

Latest revision as of 13:06, 19 October 2009

pIDTSmart-KAN Vector

This is a plasmid with a

1) pUC 18 derived origin of replication

2) Gene for kanamycin Resistance

3) M13 forward and reverse primer sites

4) Contains biobrick prefix and suffix

Snapshot of pIDT Smart-Kan vector containing YcdB gene

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 1925
Illegal suffix found in sequence at 2
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1925
Illegal SpeI site found at 3
Illegal PstI site found at 17
Illegal NotI site found at 10
Illegal NotI site found at 1931
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1925
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 1925
Illegal suffix found in sequence at 3
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 1925
Illegal XbaI site found at 1940
Illegal SpeI site found at 3
Illegal PstI site found at 17
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K233324 short</partinfo>
@@ Line 5: / Line 4: @@
 This is a plasmid with a
-) pUC 18 derived origin of replication
+<p>1) pUC 18 derived origin of replication </p>
-) Gene for kanamycin Resistance
+<p>2) Gene for kanamycin Resistance</p>
-) M13 forward and reverse primer sites (Forward primer 7-22; Reverse Primer 369-386 nu)
+<p>3) M13 forward and reverse primer sites </p>
+<p>4) Contains biobrick prefix and suffix</p>
+[[Image:Pidt smart.JPG |right|400px|thumbnail|Snapshot of pIDT Smart-Kan vector containing YcdB gene ]]
+<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
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