Difference between revisions of "Part:BBa K4619003"
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<partinfo>BBa_K4619003 short</partinfo> | <partinfo>BBa_K4619003 short</partinfo> | ||
| − | + | This part codes for TcpP ligand binding domain(TcpP-LBD). TcpP-LBD is a single-domain protein that binds the human primary bile acid and could form a dimer when bound. This process is achieved by rewiring TcpP–TcpH from *V. cholerae* to *E.coli* and fusing it with the CadC DNA binding domain to construct a chimeric receptor. When activated, TcpP-CadC forms a dimer and activates the pCadBA promoter, turning on the quorum-sensing cascade. | |
| − | + | Since TcpP LBD is a single-domain protein, it could be easily swapped into other ligand binding domains like nanobodies, promoting compatibility for the platform. | |
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Revision as of 16:35, 9 October 2023
TcpP-LBD
This part codes for TcpP ligand binding domain(TcpP-LBD). TcpP-LBD is a single-domain protein that binds the human primary bile acid and could form a dimer when bound. This process is achieved by rewiring TcpP–TcpH from *V. cholerae* to *E.coli* and fusing it with the CadC DNA binding domain to construct a chimeric receptor. When activated, TcpP-CadC forms a dimer and activates the pCadBA promoter, turning on the quorum-sensing cascade. Since TcpP LBD is a single-domain protein, it could be easily swapped into other ligand binding domains like nanobodies, promoting compatibility for the platform.
Sequence and Features No part name specified with partinfo tag.