Difference between revisions of "Part:BBa K211001:Design"

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===References===
 
===References===
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1.Radhika, V. ''et al'' . Chemical sensing of DNT by engineered olfactory yeast strain. ''Nat. Chem. Biol.'' '''3''' , 325–330 (2007.)
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2.Minic, J. ''et al''. Functional expression of olfactory receptors in yeast and development of
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a bioassay for odorant screening. ''FEBS J.'' '''272''', 524–537 (2005).

Revision as of 12:15, 19 October 2009

RI7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 834


Design Notes

Designed with PCR.

We would like to thank Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.

Source

Rat olfactory protein, clone I7 (GenBank: M64386.1)

References

1.Radhika, V. et al . Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 , 325–330 (2007.)

2.Minic, J. et al. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS J. 272, 524–537 (2005).