Difference between revisions of "Part:BBa K4897014"

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<partinfo>BBa_K4897014 short</partinfo>
 
<partinfo>BBa_K4897014 short</partinfo>
  
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This part allows the gene expression during the Dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter.  
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This part allows the gene expression during a dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter.  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 
With this part built into our blue light suicide system, once blue light is undetectable by EL222, which means a dark environment is provided at night, the formation of EL222 dimers is terminated and the RNAP is allowed to bind back to the -35 to -10 region of the luxI promoter. This thereby activates the expression of the two types of CAS9 (CAS9-A and CAS9-R) and their sgRNA sequence (sgRNA-A and sgRNA-R). This allows our target bacteria to be killed and ensures the safety of envioronment.
 
With this part built into our blue light suicide system, once blue light is undetectable by EL222, which means a dark environment is provided at night, the formation of EL222 dimers is terminated and the RNAP is allowed to bind back to the -35 to -10 region of the luxI promoter. This thereby activates the expression of the two types of CAS9 (CAS9-A and CAS9-R) and their sgRNA sequence (sgRNA-A and sgRNA-R). This allows our target bacteria to be killed and ensures the safety of envioronment.

Revision as of 15:31, 9 October 2023


LUXI promoter and EL222 binding sequence

This part allows the gene expression during a dark environment. Aiming to develop this bule-light-activated suicide system, where our engineering E. coli will be killed during the absence of blue light, namely in dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus binding to the EL222 binding region located between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter.

Usage and Biology

With this part built into our blue light suicide system, once blue light is undetectable by EL222, which means a dark environment is provided at night, the formation of EL222 dimers is terminated and the RNAP is allowed to bind back to the -35 to -10 region of the luxI promoter. This thereby activates the expression of the two types of CAS9 (CAS9-A and CAS9-R) and their sgRNA sequence (sgRNA-A and sgRNA-R). This allows our target bacteria to be killed and ensures the safety of envioronment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]