Difference between revisions of "Part:BBa K4619001"
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<partinfo>BBa_K4619001 short</partinfo> | <partinfo>BBa_K4619001 short</partinfo> | ||
| − | PobR is a protein that functions as a transcriptional activator. It first attaches to the pobR operator [https://parts.igem.org/Part: | + | PobR is a protein that functions as a transcriptional activator. It first attaches to the pobR operator [https://parts.igem.org/Part:BBa_K4271007 BBa_K4271007]on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the pobA side of the dual-directional pobA/R promoter [https://parts.igem.org/Part:BBa_K4271005 BBa_K4271005].One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage. |
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Revision as of 15:18, 9 October 2023
PobR
PobR is a protein that functions as a transcriptional activator. It first attaches to the pobR operator BBa_K4271007on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the pobA side of the dual-directional pobA/R promoter BBa_K4271005.One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 462
Illegal AgeI site found at 154
Illegal AgeI site found at 675 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 595