Difference between revisions of "Part:BBa K4591003"
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<partinfo>BBa_K4591003 short</partinfo> | <partinfo>BBa_K4591003 short</partinfo> | ||
| − | A membrane-integrating protein for membrane protein expression in Bacillus subtilis. | + | <p>A membrane-integrating protein for membrane protein expression in Bacillus subtilis.</p> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<p>Mistic（MstX） is a unique protein discovered in a select few bacterial species, including Bacillus subtilis. When fused to the N-terminus of a cargo protein construct, it enables high-level heterologous expression and targets the complete membrane protein sequence to the cell membrane. Despite its small size and high acidity, Mistic associates with membranes, likely through autonomous binding to phospholipid bilayers, thereby bypassing or facilitating conventional secretion machinery.</p> | <p>Mistic（MstX） is a unique protein discovered in a select few bacterial species, including Bacillus subtilis. When fused to the N-terminus of a cargo protein construct, it enables high-level heterologous expression and targets the complete membrane protein sequence to the cell membrane. Despite its small size and high acidity, Mistic associates with membranes, likely through autonomous binding to phospholipid bilayers, thereby bypassing or facilitating conventional secretion machinery.</p> | ||
| − | <p>The sequence and NMR structure of Mistic have been deposited in the NCBI protein database by TARMO P. | + | <p>The sequence and NMR structure of Mistic have been deposited in the NCBI protein database by TARMO P. ROOSILD et al. Matthew E. Lundberg, Eric C. Becker, Senyon Choe explored the mechanism by which MstX promotes the synthesis of the biofilm in Bacillus subtilis. Through mechanisms like regulating potassium ion channels and activating regulatory factors, MstX enhances biofilm formation in B. subtilis. </p> |
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| + | <img src="https://static.igem.wiki/teams/4591/wiki/parts/mstx1.png" width="900" height="auto"> | ||
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| + | <p style="text-align: center;">Fig 1. A kind of simple XylSmut-based fluorometric biosensors which can detect the TPA</p> | ||
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| + | <!-- Add more about the biology of this part here | ||
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Revision as of 14:30, 9 October 2023
Mistic (Mstx)
A membrane-integrating protein for membrane protein expression in Bacillus subtilis.
Usage and Biology
Mistic（MstX） is a unique protein discovered in a select few bacterial species, including Bacillus subtilis. When fused to the N-terminus of a cargo protein construct, it enables high-level heterologous expression and targets the complete membrane protein sequence to the cell membrane. Despite its small size and high acidity, Mistic associates with membranes, likely through autonomous binding to phospholipid bilayers, thereby bypassing or facilitating conventional secretion machinery.
The sequence and NMR structure of Mistic have been deposited in the NCBI protein database by TARMO P. ROOSILD et al. Matthew E. Lundberg, Eric C. Becker, Senyon Choe explored the mechanism by which MstX promotes the synthesis of the biofilm in Bacillus subtilis. Through mechanisms like regulating potassium ion channels and activating regulatory factors, MstX enhances biofilm formation in B. subtilis.
Fig 1. A kind of simple XylSmut-based fluorometric biosensors which can detect the TPA
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]