Difference between revisions of "Part:BBa K4591003"
| Line 7: | Line 7: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <p>Mistic（MstX） is a unique protein discovered in a select few bacterial species, including Bacillus subtilis. When fused to the N-terminus of a cargo protein construct, it enables high-level heterologous expression and targets the complete membrane protein sequence to the cell membrane. Despite its small size and high acidity, Mistic associates with membranes, likely through autonomous binding to phospholipid bilayers, thereby bypassing or facilitating conventional secretion machinery.</p> | ||
| + | <p>The sequence and NMR structure of Mistic have been deposited in the NCBI protein database by TARMO P. ROOSILD et al. Matthew E. Lundberg, Eric C. Becker, Senyon Choe explored the mechanism by which MstX promotes the synthesis of the biofilm in Bacillus subtilis. Through mechanisms like regulating potassium ion channels and activating regulatory factors, MstX enhances biofilm formation in B. subtilis. </p> | ||
<!-- --> | <!-- --> | ||
Revision as of 14:11, 9 October 2023
Mistic (Mstx)
A membrane-integrating protein for membrane protein expression in Bacillus subtilis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]