Difference between revisions of "Part:BBa K203105:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(2 intermediate revisions by the same user not shown)
Line 10: Line 10:
 
* We used the following primers with BBb_standard for the amplification and isolation of the LDL receptor promoter:  
 
* We used the following primers with BBb_standard for the amplification and isolation of the LDL receptor promoter:  
 
** primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTAGCCCAATTCGCCCTTGGC-3'
 
** primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTAGCCCAATTCGCCCTTGGC-3'
−
** primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCTTCGCCCTTGGCCCACGTC-3
+
** primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCTTCGCCCTTGGCCCACGTC-3'
  
 
===Source===
 
===Source===
−
 
+
Addgene, Cambridge (USA): Plasmid 14940, pLDLR-Luc (aka: pES7).
−
PCR amplified from Human genomic DNA.
+
  
 
===References===
 
===References===
 +
Castoreno, A. B. et al. Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Proc Natl Acad Sci U S A 102, 13129–13134 (2005).

Latest revision as of 10:35, 19 October 2009

LDL receptor promoter (sterol regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 139
    Illegal SapI site found at 128


Design Notes

  • The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14940, pLDLR-Luc (aka: pES7).
  • We used the following primers with BBb_standard for the amplification and isolation of the LDL receptor promoter:
    • primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTAGCCCAATTCGCCCTTGGC-3'
    • primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCTTCGCCCTTGGCCCACGTC-3'

Source

Addgene, Cambridge (USA): Plasmid 14940, pLDLR-Luc (aka: pES7).

References

Castoreno, A. B. et al. Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Proc Natl Acad Sci U S A 102, 13129–13134 (2005).