Difference between revisions of "Part:BBa K4765101"

(→‎Usage and Biology)
(→‎Usage and Biology)
Line 7: Line 7:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
Similar to [https://parts.igem.org/Part:BBa_K4765103 BBa_K4765103(ribozyme+strong RBS+intimin-Ag2 fusion+stem-loop)], we replaced Ag2 with Ag1 in order to interact with [https://parts.igem.org/Part:BBa_K4765102 BBa_K4765102(ribozyme+strong RBS+intimin-Nb1 fusion+stem-loop)] to create a biofilm.
+
Similar to [https://parts.igem.org/Part:BBa_K4765105 BBa_K4765105], we replaced Ag3 with Ag1 in order to interact with [https://parts.igem.org/Part:BBa_K4765102 BBa_K4765102] to create a biofilm.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:40, 9 October 2023


Twister P1 + T7_RBS + intimin-Ag1 fusion + stem-loop

contributed by Fudan iGEM 2023

Usage and Biology

Similar to BBa_K4765105, we replaced Ag3 with Ag1 in order to interact with BBa_K4765102 to create a biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305
    Illegal SapI.rc site found at 2375