Difference between revisions of "Part:BBa K203106"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | 3-hydroxy-3-methylglutaryl-CoA synthase (HMG CoA synthase) is an enzyme catalyzing the condensation of Acetyl-CoA and acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). There are two distinct HMG CoA synthases, the cytosolic and the mitochondrial form, encoded by two different genes. The reaction catalyzed by the cytosolic enzyme is a part of the biosynthesis of cholesterol. In mitochondria the same reaction is responsible for keton body formation. Sterol regulatory elements in the promoter region of the gene are responsible for transcriptional regulation [ | + | 3-hydroxy-3-methylglutaryl-CoA synthase (HMG CoA synthase) is an enzyme catalyzing the condensation of Acetyl-CoA and acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). There are two distinct HMG CoA synthases, the cytosolic and the mitochondrial form, encoded by two different genes. The reaction catalyzed by the cytosolic enzyme is a part of the biosynthesis of cholesterol. In mitochondria the same reaction is responsible for keton body formation. Sterol regulatory elements in the promoter region of the gene are responsible for transcriptional regulation. See reference at [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#Proteins Eucaryopedia]. |
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<partinfo>BBa_K203106 SequenceAndFeatures</partinfo> | <partinfo>BBa_K203106 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K203106 parameters</partinfo> | <partinfo>BBa_K203106 parameters</partinfo> | ||
Revision as of 09:10, 19 October 2009
HMG-CoA synthase promoter (sterol regulated)
This part is a natural promoter responsive to...... and when placed upstream of a gene makes it regulatable by the elements to which this promoter is sensitive.
Usage and Biology
3-hydroxy-3-methylglutaryl-CoA synthase (HMG CoA synthase) is an enzyme catalyzing the condensation of Acetyl-CoA and acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). There are two distinct HMG CoA synthases, the cytosolic and the mitochondrial form, encoded by two different genes. The reaction catalyzed by the cytosolic enzyme is a part of the biosynthesis of cholesterol. In mitochondria the same reaction is responsible for keton body formation. Sterol regulatory elements in the promoter region of the gene are responsible for transcriptional regulation. See reference at [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#Proteins Eucaryopedia].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| n/a | HMG-CoA synthase promoter (sterol regulated) |
| [[Image:|thumb|none|350px|BBa_K203106 is a natural HMG CoA synthase promoter induced approximately by ?% by Lipoprotein Deficient Serum;. [http://2009.igem.org/Team:Heidelberg/Measurement REU] was determined by TECAN, 1 experiment à three replicates]] |