Difference between revisions of "Part:BBa K4765113"
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===Characterization=== | ===Characterization=== | ||
− | + | Get details in [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016]. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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==Reference== | ==Reference== |
Revision as of 08:48, 9 October 2023
Twister P1 + T7_RBS + H. ex mtSSB + stem-loop
Introduction
This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and ribozyme-assisted polycistronic co-expression system:pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.
Usage and Biology
We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with BBa_K4765112(ribozyme+T7_RBS+SAHS 33020+stem-loop) to increase the survival rate of E.coli under desiccation.
Characterization
Get details in BBa_K4765016. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409