Difference between revisions of "Part:BBa K4814012"
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To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter by BBa_K3020000, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. | To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter by BBa_K3020000, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. | ||
Sequence: TTTAAGAGGGGGCTATACAT | Sequence: TTTAAGAGGGGGCTATACAT | ||
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+ | <html> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-uvb.png alt=""style="width:400px;height:auto;"> | ||
+ | </td> | ||
+ | <td> | ||
+ | <img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-h2o2.png alt=""style="width:400px;height:auto;"> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p>RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-na.png alt=""style="width:400px;height:auto;"> | ||
+ | </td> | ||
+ | <td> | ||
+ | <img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-ap-1.png alt=""style="width:400px;height:auto;"> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p>RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p> | ||
+ | </html> | ||
References: | References: |
Revision as of 08:45, 9 October 2023
Strong RBS
To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter by BBa_K3020000, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. Sequence: TTTAAGAGGGGGCTATACAT
RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.
RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.
References:
Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]