Difference between revisions of "Part:BBa K4814012"

 
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To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter by BBa_K3020000, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates.  
 
To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter by BBa_K3020000, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates.  
 
Sequence: TTTAAGAGGGGGCTATACAT
 
Sequence: TTTAAGAGGGGGCTATACAT
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-uvb.png alt=""style="width:400px;height:auto;">
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-h2o2.png alt=""style="width:400px;height:auto;">
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<p>RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-na.png alt=""style="width:400px;height:auto;">
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-ap-1.png alt=""style="width:400px;height:auto;">
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<p>RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
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References:
 
References:

Revision as of 08:45, 9 October 2023


Strong RBS

To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter by BBa_K3020000, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. Sequence: TTTAAGAGGGGGCTATACAT

RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.

RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.

References:

Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]